Diminution of Proliferation, Migration, and Invasion of Melanoma Cells by Arctium Lappa L. Extracts.

Citation metadata

Date: May 2021
Publisher: New Century Health Publishers, LLC
Document Type: Article
Length: 4,247 words
Lexile Measure: 1340L

Document controls

Main content

Abstract :

As a highly aggressive form of human cancer, melanoma is prone fo metastasis, leading to 75% of all skin cancer deaths. The 5-year-survival rate of melanoma is below 20%. Therefore, new therapies for melanoma are urgently needed. Burdock (Arctium lappa L) root - known for its antitumor, antioxidant, anti-inflammatory, antiviral, neuroprotective, and endoplasmic reticulum stress regulatory effects - was evaluated for its effects on the proliferation and apoptosis of melanoma cells and its possible mechanism. The results show suppression of the proliferation and stimulation of apoptosis of A375 cells in vitro. Additionally, it suppressed the PI3K/AKT pathway in A375 cells. These data suggest burdock (A. lappa L) root preparation as a promising drug for the treatment of melanoma. Keywords: Apoptosis, Arctium lappa L, Melanoma, PI3K/AKT pathway, Proliferation Abbreviations Used: Protein kinase B, AKT; Arctium lappa L, ALHE; Cyclin-dependent kinase 4, CDK4; Dulbecco's modified eagle medium, DMEM; Dimethyl sulfoxide, DMSO; Enhanced chemiluminescence, ECL; Endoplasmic reticulum, ER; Fetal bovine serum, FBS; Glyceraldehyde-3-phosphate dehydrogenase, GAPDH; Horse radish peroxidase, HRP; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, MTT; Phosphoprotein kinase B, pAKT; Phosphate-buffered saline, PBS; Paraformaldehyde, PFA; Propidium iodide, PI; Phosphoinositide-3-kinase, PI3K; Phospho-phosphoinositide-3-kinase, pPI3K; Polyvinylidene difluoride, PVDF; Sodium dodecyl sulfate polyacrylamide gel-electrophoresis, SDS-PAGE; Tris-buffered saline, TBST Corresponding Author: Dr. Yulei Liu, Department of Dermatology, The Affiliated Hospital of Inner Mongolia Medical University, No.1 Tongdao North Road, Huimin District, Hohhot, Inner Mongolia Autonomous Region, China; E-mail: ylliu4856@163.com

Source Citation

Source Citation   

Gale Document Number: GALE|A659273152