A scalable high-throughput targeted next-generation sequencing assay for comprehensive genomic profiling of solid tumors.

Citation metadata

From: PLoS ONE(Vol. 16, Issue 12)
Publisher: Public Library of Science
Document Type: Report
Length: 14,032 words
Lexile Measure: 1660L

Document controls

Main content

Abstract :

Timely and accurate identification of molecular alterations in solid tumors is essential for proper management of patients with advanced cancers. This has created a need for rapid, scalable comprehensive genomic profiling (CGP) systems that detect an increasing number of therapeutically-relevant variant types and molecular signatures. In this study, we assessed the analytical performance of the TruSight Oncology 500 High-Throughput assay for detection of somatic alterations from formalin-fixed paraffin-embedded tissue specimens. In parallel, we developed supporting software and automated sample preparation systems designed to process up to 70 clinical samples in a single NovaSeq 6000.sup.TM sequencing run with a turnaround time of 99% overall accuracy and precision. Our results demonstrate that the high-throughput CGP assay is a reliable method for accurate detection of molecular alterations in support of precision therapeutics in oncology. The supporting systems and scalable workflow allow for efficient interpretation and prompt reporting of hundreds of patient cancer genomes per week with excellent analytical performance.

Source Citation

Source Citation   

Gale Document Number: GALE|A684621222