Abstract :
Byline: Benjamin J. Gomez-Moliner, Arantzazu M. Elejalde, Jose R. Arrebola, Ana I. Puente, Alberto Martinez-Orti, Antonio Ruiz, Maria J. Madeira Gomez-Moliner, B.J., Elejalde, A.M., Arrebola, J.R., Puente, A.I., Martinez-Orti, A., Ruiz, A. & Madeira, MJ. (2012). Molecular phylogeny of the Helicodontidae and Trissexodontidae (Gastropoda). -Zoologica Scripta, 00, 000-000. In this study, we present a molecular phylogeny of the Trissexodontidae and Helicodontidae obtained by means of Maximum Parsimony, Neighbor Joining, Maximum Likelihood and Bayesian analyses of DNA sequences. Nearly 3 KB of sequence data of two mitochondrial genes (COI, 16S rDNA) and the nuclear rRNA gene cluster including ITS-1, the 3a[sup.2]end of the 5.8S gene, the complete ITS-2 region and 5a[sup.2] end of the large subunit 28S were used to reconstruct the phylogeny of these two families. Monophyly of Trissexodontidae and Helicodontidae at the family level is well supported. A new classification of the genera in the Trissexodontidae is proposed. It includes two subfamilies: Gittenbergeriinae (monotypic for Gittenbergeria turriplana) and Trissexodontinae. The latter includes three strongly supported tribes: (i) Trissexodontini, including Mastigophallus, Trissexodon, Oestophorella and Suboestophora; (ii) Oestophorini, with Oestophora; and (iii) Caracollinini, with Caracollina, Gasulliella, Gasullia and Hatumia. The polytypic Oestophora and Suboestophora are recovered as two monophyletic genera. The anatomy of the auxiliary copulatory organs of the reproductive system is coherent with the new taxonomic interpretation of the Trissexodontidae. Further work, including some more taxa is needed to delimitate subfamilies within Helicodontidae. Finally, the addition of some sequences of other Helicoidea shows that the genus Ciliella is not closely related to Trissexodontidae, being grouped within the Hygromiidae, instead. Correspondence: (*) Corresponding author: Maria J. Madeira, Department of Zoology and Animal Cell Biology, Faculty of Pharmacy, University of the Basque Country, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Alava, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006, Alava, Spain. E-mail: mariajose.madeira@ehu.es Arantzazu M. Elejalde, Department of Zoology and Animal Cell Biology, Faculty of Pharmacy, University of the Basque Country, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Alava, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006, Alava, Spain. E-mail: mirenarantzazu.elejalde@ehu.es Jose R. Arrebola, Department of Animal Biology (Zoology), Faculty of Biology, University of Seville, Avda. Reina Mercedes, 6. 41012, Seville, Spain. E-mail: mastus@us.es Ana I. Puente, Department of Zoology and Animal Cell Biology, Faculty of Sciences and Technology, University of the Basque Country, Barrio Sarriena s/n, 48940 Leioa, Bizkaia, Spain. E-mail: ana.puente@ehu.es Alberto Martinez-Orti, Department of Zoology, Faculty of Biological Sciences, University of Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain. E-mail: alberto.martinez@uv.es Antonio Ruiz, Department of Animal Biology (Zoology), Faculty of Biology, University of Seville, Avda. Reina Mercedes, 6. 41012, Seville, Spain. E-mail: antoniocursospesca@gmail.com Benjamin J. Gomez-Moliner, Department of Zoology and Animal Cell Biology, Faculty of Pharmacy, University of the Basque Country, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Alava, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006, Alava, Spain. E-mail: benjamin.gomez@ehu.es Submitted: 12 April 2012 Accepted: 15 September 2012 Supporting information: Additional Supporting Information may be found in the online version of this article Fig. S1. Alignment of the mitochondrial and nuclear ribosomal gene fragments (COI, 16S, ITS 2-28S and ITS 1), including the indels considered in the analysis. Table S1. List of the species used in this study for mitochondrial and nuclear gene fragments, indicating sampling localities, geographical coordinates (UTM) and GenBank accession numbers. Asterisks (*) indicate the sequences obtained from GenBank. Table S2. List of primers used for amplification and sequencing.