Preliminary study about sublingual administration of bacteria-expressed pandemic H1N1 influenza vaccine in miniature pigs

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From: The Journal of Microbiology(Vol. 52, Issue 9)
Publisher: Springer
Document Type: Report
Length: 377 words

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Byline: Hyekwon Kim (1), Jeong-Ki Kim (2), Hohyun Song (3), Jungah Choi (3), Byoungshik Shim (3), Bokyu Kang (4), Hyoungjoon Moon (4), Minjoo Yeom (5), Sang-Hyun Kim (5), Daesub Song (5), Manki Song (3) Keywords: pandemic; influenza; HA1; sublingual vaccine; pig Abstract: Sublingual (SL) administration of influenza vaccine would be non-invasive and effective way to give human populations protective immunity against the virus, especially when pandemic influenza outbreaks. In this study, the efficacy of pandemic influenza virus-based subunit vaccines was tested after sublingual (SL) adjuvant administration in pigs. Eight specific pathogen-free Yucatan pigs were divided into 4 groups: nonvaccinated but challenged (A) and vaccinated and challenged (B, C, and D). The vaccinated groups were subdivided by vaccine type and inoculation route: SL subunit vaccine (hemagglutinin antigen 1 [HA1] + wild-type cholera toxin [wtCT], B) IM subunit vaccine (HA1 + aluminum hydroxide, C) and IM inactivated vaccine (+ aluminum hydroxide, D). The vaccines were administered twice at a 2-week interval. All pigs were challenged with pandemic influenza virus (A/swine/GCVP-KS01/2009 [H1N1]) and monitored for clinical signs, serology, viral shedding, and histopathology. After vaccination, hemagglutination inhibition titre was higher in group D (320) than in the other vaccinated groups (40--80) at the time of challenge. The mobility and feed intake were reduced in group C. Both viral shedding and histopathological lesions were reduced in groups B and D. Although this study has limitation due to the limited number of pigs (2 pigs per a group), the preliminary data in this study provided the protective potential of SL administration of bacteria-expressed pandemic H1N1 influenza vaccine in pigs. There should be additional animal studies about effective adjuvant system and vaccine types for the use of SL influenza vaccination. Author Affiliation: (1) Research Evaluation Team, Institute for Basic Science, Daejeon, 305-811, Republic of Korea (2) College of Pharmacy, Korea University, Sejong, 339-700, Republic of Korea (3) Molecular Vaccinology Section, Laboratory Science Division, International Vaccine Institute, Seoul, 151-919, Republic of Korea (4) Research Unit, Green Cross Veterinary Products, Yong-in, 449-903, Republic of Korea (5) Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejon, 305-806, Republic of Korea Article History: Registration Date: 29/07/2014 Received Date: 15/05/2014 Accepted Date: 16/06/2014 Online Date: 30/07/2014 Article note: These authors contributed equally to the study

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Gale Document Number: GALE|A382506527