Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples.

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Publisher: Hindawi Limited
Document Type: Article
Length: 5,787 words
Lexile Measure: 1070L

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Abstract :

Amyloid beta peptides (Aβ 1-42) have been found to be associated with the cause of Alzheimer's disease (AD) and dementia. Currently, methods for detecting Aβ 1-42 are complicated and expensive. The present study is aimed at developing an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) to detect Aβ 1-42 by using a polyclonal antibody from alpaca, an application used in urine samples. The serum was collected from the alpaca after immunizing it with Aβ 1-42 at 500μ g/injection 5 times. The ic-ELISA was developed and showed a half-maximal inhibitory concentration (IC[sub.50]) of 103.20ng/ml. The limit of detection (LOD) was 0.39ng/100μ l. The cross-reactivity was tested with Aβ 1-40 and 8 synthesized peptides that had sequence similarities to parts of Aβ 1-42. The cross-reactivity of Aβ 1-40 and peptide 1 (DAEFRHDSGYE) was 55% and 69.4%, respectively. The ic-ELISA was applied to analyze Aβ 1-42 in the urine and precipitated protein urine samples. This method can be used for detecting a normal level of total soluble Aβ (approximately 1ng in 5mg of precipitated urine protein) and can be used for detecting the early stages of AD. It is considered to be an easy and inexpensive method for monitoring and diagnosing AD.

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Gale Document Number: GALE|A695445318