Determination of T Cell Responses in Thai Systemic Sclerosis Patients.

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Publisher: Hindawi Limited
Document Type: Article
Length: 6,305 words
Lexile Measure: 1270L

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Abstract :

Objectives. This study is aimed at determining the role of T cells by assessing the numbers of IFN-γ - and IL-2-secreting T cells following stimulation with peptides derived from DNA topoisomerase-I protein in Thai SSc patients. Methods. Fifty Thai SSc patients and 50 healthy controls (HC) joined this study. IFN-γ and IL-2 levels upon stimulation of T cells with 6 peptides derived from DNA topoisomerase-I protein were determined. Anti-nuclear antibodies (ANA) and anti-Scl-70 antibodies were determined by using the ELISA method. Results. In SSc patients, we detected a significantly higher number of IFN-γ - and IL-2-secreting CD8[sup.+] T cells than IFN-γ - and IL-2-secreting CD4[sup.+] T cells after stimulation with pooled peptides derived from DNA topoisomerase-I protein. A similar percentage of CD4[sup.+]IL-2[sup.+], CD4[sup.+]IFN-γ [sup.+], and CD8[sup.+]IL-2[sup.+] were detected following stimulation with DNA topoisomerase-I protein -in SSc patients with anti-Scl-70 antibody (SSc/anti-Scl-70[sup.+]) and those without. In contrast, the amount of CD8[sup.+]IFN-γ [sup.+] cells was significantly higher in SSc/anti-Scl-70[sup.+] than those without. Stimulation with individual peptides showed that CSLRVEHINLHPELD (sPep3; 15 amino acids; position 505-519 of DNA topoisomerase-I protein) was the optimal epitope that induced T cells secreting the highest levels of IFN-γ and IL-2. A higher percentage of IFN-γ [sup.+]CD4[sup.+] T cells was detected in SSc/anti-Scl-70[sup.+] than those without the following stimulation with peptides 2 (amino acid position 475-486 [RAVALYFIDKLA] of protein DNA topoisomerase). Conclusion. The results from this study emphasize the critical role of DNA topoisomerase-I peptides on the activation of T cells in SSc patients. The findings provide a better understanding of SSc's immunopathogenesis and may lead to the development of diagnostic tools and specific treatments for SSc in the future.

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Gale Document Number: GALE|A697641504