Crude extract of whole plant of Momordica charantia was made to go through fractionation processes using thin layer chromatography (TLC) and the resultant fractions were tested for antibacterial activity against four pathogens. Chromatographic analysis yielded yellow fraction 690mg, dark green fraction 570mg while 740mg of the blue black fraction was obtained. At the concentration of about 40[micro]g/ml fraction 1 (yellow fraction) was active against all test organisms except Salmonella typhi, but at the same concentration fraction 2 (dark green fraction) was active against two of the four test organisms Staphylococcus aureus and Streptococcus pyogenes while the third fraction (blue black fraction) was active against only one organism Streptococcus pyogenes. Results of Minimum Inhibitory Concentration (MIC) studies showed MIC between 40[micro]g/ml and 60[micro]g/ml for fractions 1 and 2 but 40[micro]g/ml and 80[micro]g/ml for fraction 3, while Minimum Bactericidal Concentration (MBC) studies revealed that MBC ranged from 40[micro]g/ml-80[micro]g/ml for fractions 1 and 2 but 60[micro]g/ml-100[micro]g/ml for fraction 3. The three fractions were active against the test organisms showing that a useful drug could be developed from this plant against some bacterial pathogens. Key words: antibacterial activity, chromatographic fractions, whole plant, Momordica charantia.