To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1007/s12272-017-0913-2 Byline: Hong Guo (1), Zisong Bai (1,3), Yangyang Xu (1), Xueqin Wu (1,3), Ning Li (2), Yan Zhu (1), Xiaoming Wang (1), Peng Zhang (1) Keywords: Furanoflavone; Anti-inflammation; Pongamia pinnata (Linn.) Pierre; Bioactivities guided isolation Abstract: Pongamia pinnata (Linn.) Pierre has anti-inflammatory activity and could significantly decrease serum tumor necrosis factor-[alpha] and IL-10 in arthritic rats. Previous research indicated the typical chemical constituent in P. pinnata is furanoflavone. Guided by anti-inflammatory active assay and UPLC-HRESIMS chromatography, 22 compounds were isolated from the ethanol extract of P. pinnata seedpods. One novel furanoflavone, 4 -hydroxypinnatin, was elucidated by HRESIMS, 1D- and 2D-NMR spectra. The 21 known compounds, including 9 furanoflavone, were identified by comparing their NMR data with the previous data in reference. In the known compounds, 5 were isolated for the first time from the species. The anti-inflammatory activities were assayed by assessing LPS-induced NO production in BV-2 cells. 12 compounds can inhibit the production of NO without cytotoxicity at concentration of 50 [micro]M. Among them, compounds 4 can significantly inhibit the production of NO, with the IC.sub.50 value of 31.36 [micro]M. Author Affiliation: (1) Tianjin State Key Laboratory of Modern Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People's Republic of China (2) School of Traditional Chinese Materia Medica Key Laboratory of Structure-Based Drug Design and Discovery, Shenyang Pharmaceutical University, Ministry of Education, Wenhua Road 103, Shenyang, 110016, People's Republic of China (3) Tianjin International Joint Academy of Biomedicine, Tianjin, 300457, People's Republic of China Article History: Registration Date: 28/03/2017 Received Date: 08/11/2016 Accepted Date: 27/03/2017 Online Date: 25/05/2017 Article note: Hong Guo and Zisong Bai have equally contributed to this work.