Investigating the Dynamics of Destabilized Nucleosomes Using Methyl-TROSY NMR

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From: Journal of the American Chemical Society(Vol. 140, Issue 14)
Publisher: American Chemical Society
Document Type: Report
Length: 67 words

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Abstract :

Using methyl-TROSY NMR approaches, the effect of point mutations designed, is evaluated to perturb key histone interfaces which become destabilized during nucleosome remodeling in an effort to probe nucleosome core particle (NCP) plasticity. Notably the NCP retains its overall structural integrity. This work highlights the inherent plasticity of NCPs and establishes methyl-TROSY NMR as a valuable compliment to current single molecule methods in quantifying NCP dynamic properties.

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Gale Document Number: GALE|A540752473