A heating-superfusion platform technology for the investigation of protein function in single cells

Citation metadata

From: Analytical Chemistry(Vol. 87, Issue 1)
Publisher: American Chemical Society
Document Type: Report
Length: 77 words

Document controls

Main content

Abstract :

The article describes a novel approach for the study of single-cell intracellular enzyme activity at various temperatures, utilizing a localized laser heating probe in combination with a freely positionable microfluidic perfusion device. Quantitative estimates for the intracellular alkaline phosphatase activity were generated at five different temperatures in different cell lines for constructing temperature-response curves of enzymatic activity at the single-cell level. After cell permeation, enzymatic activity was determined rapidly, generating five-point temperature-response curves within just 200 s.

Source Citation

Source Citation   

Gale Document Number: GALE|A402150950