To access, purchase, authenticate, or subscribe to the full-text of this article, please visit this link: http://dx.doi.org/10.1016/j.jped.2016.06.011 Byline: Caroline Ayres [email@example.com] (a,b,*), Charles F. Ferreira (a,b,c), Juliana R. Bernardi (a,b,d), Thiago B. Marcelino (a,b), Vania N. Hirakata (e), Clecio H. da Silva (a,b), Marcelo Z. Goldani (a,b) Keywords Newborn; Facial expression; Sucrose Abstract Objective This study describes a quantitative and qualitative methodology to assess hedonic responses to sweet stimulus in healthy newborns. Methods A descriptive, cross-sectional, observational study, with healthy newborns (up to 24 h of life), between 37 and 42 gestational weeks, vaginally born and breastfed previously to all tests. The evaluation of the newborns reactions was performed by hedonic facial expression analysis, characterized by facial expressions with rhythmic serial tongue protrusion after neutral or sweet solution intake. Initially, 1 mL of water solution was provided to the newborn, followed by a 1-minute recording. Afterwards, the same amount of 25% sucrose solution was provided, performing a second recording. The concordance between researchers was analyzed by the Bland--Altman statistical method. Results A total of 100 newborns (n= 49 males, n= 51 females; mean lifetime = 15 h 12 min [plus or minus] 6 h 29 min) were recorded for neutral and sucrose solution intake, totaling 197 videos (n= 3 missing in the water treatment). These videos were double-blind analyzed and the test revealed a 90% concordance between the two trained researchers, in relation to both solutions. The intraclass correlation coefficient was 0.99 for both solutions, with a significant increase in frequency of hedonic expressions evoked by sucrose solution intake. Conclusions These results confirm that the proposed method has an efficient power to detect significant differences between neutral and sucrose stimuli. In conclusion, this evaluation method of hedonic facial reactions in newborns reflects the response to a specific taste. Author Affiliation: (a) Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Nucleo de Estudos em Saude da Crianca e do Adolescente (NESCA), Porto Alegre, RS, Brazil (b) Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Pos-Graduacao em Saude da Crianca e do Adolescente, Porto Alegre, RS, Brazil (c) Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Pos-Graduacao em CiA*ncias da Saude: Ginecologia e Obstetricia (PPGGO), Porto Alegre, RS, Brazil (d) Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Departamento de Nutricao, Porto Alegre, RS, Brazil (e) Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Grupo de Pesquisa e Pos-Graduacao (GPPG), Porto Alegre, RS, Brazil * Corresponding author. Article History: Received 25 November 2015; Accepted 20 June 2016 (footnote)[white star] Please cite this article as: Ayres C, Ferreira CF, Bernardi JR, Marcelino TB, Hirakata VN, Silva CH, et al. A method for the assessment of facial hedonic reactions in newborns. J Pediatr (Rio J). 2017;93:253--9.