A New Extracellular [beta]-Galactosidase Producing Kluyveromyces sp. PCH397 from Yak Milk and Its Applications for Lactose Hydrolysis and Prebiotics Synthesis.

Citation metadata

From: Indian Journal of Microbiology(Vol. 61, Issue 3)
Publisher: Springer
Document Type: Report; Brief article
Length: 284 words

Document controls

Main content

Abstract :

Keywords: Keyword [beta]-Galactosidase; Lactulose; Galacto-oligosaccharides; Fructo-oligosaccharides; Milk-microbes Abstract [beta]-Galactosidase is a crucial glycoside hydrolase enzyme with potential applications in the dairy, food, and pharmaceutical industries. The enzyme is produced in the intracellular environment by bacteria and yeast. The present study reports yeast Kluyveromyces sp. PCH397 isolated from yak milk, which has displayed extracellular [beta]-galactosidase activity in cell-free supernatant through the growth phase. To investigate further, cell counting and methylene blue staining of culture collected at different growth stages were performed and suggested for possible autolysis or cell lysis, thereby releasing enzymes into the extracellular medium. The maximum enzyme production (9.94±2.53U/ml) was achieved at 37 °C in a modified deMan, Rogosa, and Sharpe (MRS) medium supplemented with lactose (1.5%) as a carbon source. The enzyme showed activity at a wide temperature range (4--50 °C), maximum at 50 °C in neutral pH (7.0). In addition to the hydrolysis of lactose (5.0%), crude [beta]-galactosidase also synthesized vital prebiotics (i.e., lactulose and galacto-oligosaccharides (GOS)). Additionally, [beta]-fructofuranosidase (FFase) activity in the culture supernatant ensued the synthesis of a significant prebiotic, fructo-oligosaccharides (FOS). Hence, the unique features such as extracellular enzymes production, efficient lactose hydrolysis, and broad temperature functionality by yeast isolate PCH397 are of industrial relevance. In conclusion, the present study unrevealed for the first time, extracellular production of [beta]-galactosidase from a new yeast source and its applications in milk lactose hydrolysis and synthesis of valuable prebiotics of industrial importance. Author Affiliation: (1) Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Himachal Pradesh, Post Box No. 6, 176 061, Palampur, India (2) Department of Microbiology, Guru Nanak Dev University, 143005, Amritsar, Punjab, India (e) dharamsingh@ihbt.res.in Article History: Registration Date: 06/08/2021 Received Date: 02/13/2021 Accepted Date: 06/08/2021 Online Date: 06/16/2021 Byline:

Source Citation

Source Citation   

Gale Document Number: GALE|A667783864