Intersex (ix) mutations of Drosophila melanogaster cause nonrandom cell death in genital disc and can induce tumours in genitals in response to decapentaplegic (dpp.sup.disk) mutations

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From: Journal of Genetics(Vol. 94, Issue 2)
Publisher: Springer
Document Type: Report
Length: 371 words

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Byline: R. N. CHATTERJEE (1), P. CHATTERJEE (1), S. KUTHE (1), M. ACHARYYA-ARI (1), R. CHATTERJEE (3) Keywords: tumour; intersex; dpp signalling; Drosophila melanogaster Abstract: In Drosophila melanogaster, the intersex (ix) is a terminally positioned gene in somatic sex determination hierarchy and function with the female specific product of double sex (DSX .sup.F) to implement female sexual differentiation. The null phenotype of ix is to transform diplo-X individuals into intersexes while leaving haplo-X animals unaffected. This study on the effect of different intersexmutations on genital disc development provides the following major results: (i) similar range of a characteristic array of morphological structures (from almost double sex terminalia to extreme reduction of terminal appendages) was displayed by the terminalia of XX ix .sup.1 /ix .sup.1, XX ix .sup.2 /ix .sup.2 and XX ix .sup.5 /ix .sup.5 individuals (ii) an increased number of apoptotic cells were found to occur in a localized manner in mature third instar larval genital discs of ix individuals (iii) ix mutations can induce high frequency of neoplastic tumours in genitals in the presence of decapentaplegic (dpp .sup.disk) mutations and (iv) heteroallelic combinations of dpp .sup.disk mutations can also induce tumours in intersex genitals with variable expressivity. On the basis of these findings, we suggest that: (i) loss of function of ixcauses massive cell death in both male and female genital primordia of genital discs, resulting phenotype mimicking in male and female characteristics in genitals and (ii) at the discs, the apoptotic cells persist as 'undead' cells that can induce oncogenic transformation in the neighbouring disc cells when dppsignalling is blocked or reduced by dpp .sup.disk mutations. Author Affiliation: (1) Genetics Research Unit, Department of Zoology, University of Calcutta, 35 B.C. Road, Kolkata, 700 019, India (2) Gabberia Girl's High School, South 24 Parganas, Gabberia, 743 336, India (3) K. P. C. Medical College, Jadavpur, Kolkata, 700 032, India Article History: Registration Date: 08/04/2015 Received Date: 06/11/2014 Accepted Date: 14/11/2014 Online Date: 21/05/2015 Article note: [Chatterjee R. N., Chatterjee P., Kuthe S., Acharyya-Ari M. and Chatterjee R. 2015 Intersex (ix) mutations of Drosophila melanogaster cause nonrandom cell death in genital disc and can induce tumours in genitals in response to decapentaplegic (dpp disk ) mutations. J. Genet. 94, xx--xx]

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Gale Document Number: GALE|A422800267