Microbiome profiling of drinking water in relation to incidence of inflammatory bowel disease

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From: Canadian Journal of Microbiology(Vol. 62, Issue 9)
Publisher: NRC Research Press
Document Type: Report
Length: 6,786 words
Lexile Measure: 1270L

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Abstract :

The etiology of inflammatory bowel disease (IBD) is unknown; current research is focused on determining environmental factors. One consideration is drinking water: water systems harbour considerable microbial diversity, with bacterial concentrations estimated at 106-108 cells/L. Perhaps differences in microbial ecology of water sources may impact differential incidence rates of IBD. Regions of Manitoba were geographically mapped according to incidence rates of IBD and identified as high (HIA) or low (LIA) incidence areas. Bulk water, filter material, and pipe wall samples were collected from public buildings in different jurisdictions and their population structure analyzed using 16S rDNA sequencing. At the phylum level, Proteobacteria were observed significantly less frequently (P = 0.02) in HIA versus LIA. The abundance of Proteobacteria was also found to vary according to water treatment distribution networks. Gammaproteobacteria was the most abundant class of bacteria and was observed more frequently (P = 0.006) in LIA. At the genus level, microbes found to associate with HIA include Bradyrhizobium (P = 0.02) and Pseudomonas (P = 0.02). Particular microbes were found to associate with LIA or HIA, based on sample location and (or) type. This work lays out a basis for further studies exploring water as a potential environmental source for IBD triggers. Keywords: inflammatory bowel disease, 16S rDNA, water microbiome, etiology, epidemiology. L'etiologie de la maladie intestinale inflammatoire (MII) est obscure; les recherches actuelles s'emploient donc a cerner les facteurs environnementaux. L'un les elements suspects est l'eau potable. Or, les systemes d'aqueducs renferment une variete considerable de microbes, soit des densites bacteriennes estimees a 106 a 108 cellules/L. On avance que des differences dans l'ecologie bacterienne des sources d'eau pourraient avoir un impact sur les taux d'incidence variables de MII. On a cartographie des regions du Manitoba selon leur taux d'incidence de MII et on a identifie des zones a incidence elevee (ZIE) et a incidence basse (ZIB). On a preleve des echantillons d'eau en vrac, de matiere de filtres et de parois de tuyaux retrouves dans des batiments publics des divers territoires, et on a analyse leur structure demographique au moyen du sequencage de l'ADNr 16S. A l'echelle du phylum, on a observe significativement (P = 0,02) moins de proteobacteries dans les ZIE que dans les ZIB. On a egalement constate que l'abondance des proteobacteries variait en fonction des reseaux de distribution et de traitement des eaux. Les gammaproteobacteries formaient la classe de bacteries la plus abondante et a ete observee plus souvent (P = 0,006) dans les ZIB. A l'echelle du genre, les microbes associes davantage aux ZIE regroupaient, entre autres, Bradyrhizobium (P = 0,02) et Pseudomonas (P = 0,02). On a constate que des microbes particuliers s'associant aux ZIE et aux ZIB, selon l'emplacement ou le type d'echantillon. Ces travaux jettent les bases d'etudes ulterieures explorant l'eau a titre de source environnementale d'elements declenchant la MII. [Traduit par la Redaction] Mots-cles : maladie intestinale inflammatoire, ADNr 16S, microbiome de l'eau, etiologie, epidemiologie.

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Gale Document Number: GALE|A462507971