Synbiotics for Broiler Chickens-In Vitro Design and Evaluation of the Influence on Host and Selected Microbiota Populations following In Ovo Delivery

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From: PLoS ONE(Vol. 12, Issue 1)
Publisher: Public Library of Science
Document Type: Report
Length: 9,468 words
Lexile Measure: 1370L

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Abstract :

Synbiotics are synergistic combinations of prebiotics and probiotics. In chickens, synbiotics can be delivered in ovo to expedite colonization of the gut by beneficial bacteria. We therefore aimed to design synbiotics in vitro and validate them in broiler chickens upon in ovo delivery. The probiotic components of the synbiotics were Lactobacillus salivarius and Lactobacillus plantarum. Their growth was assessed in MRS medium supplemented with different prebiotics. Based on in vitro results (hatchability and growth curve), two synbiotics were designed: S1 -Lactobacillus salivarius with galactooligosaccarides (GOS) and S2 -Lactobacillus plantarum with raffinose family oligosaccharides (RFO). These synbiotics were delivered to Cobb broiler chicken embryos on day 12 of incubation at optimized doses (10.sup.5 cfu egg.sup.-1 of probiotic, 2 mg egg.sup.-1 of prebiotic). Post hatching, 2,400 roosters were reared (600 individuals group.sup.-1 divided into eight replicate pens). Microbial communities were analyzed in ileal and cecal digesta on day 21 using FISH. Gene expression analysis (IL1[beta], IL4, IL6, IL8, IL12, IL18, IFN[beta], and IFN[gamma]) was performed on days 7, 14, 21, and 42 for the spleen and cecal tonsils with RT-qPCR. Body weight and feed intake of the roosters did not differ by the treatments. Microbial populations of Lactobacillus spp. and Enterococcus spp. in the ileum were higher in S1 and S2 than in the control. In the cecum, the control had the highest bacterial counts. S1 caused significant up-regulation of IL6, IL18, IL1[beta], IFN[gamma], and IFN[beta] in the spleen on day 21 and IL1[beta] on day 7 (P

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Gale Document Number: GALE|A476237525