The hepatoprotective effects of Pyrus biossieriana buhse leaf extract on tert-butyl hydroperoxide toxicity in HepG2 cell line.

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From: BMC Research Notes(Vol. 14, Issue 1)
Publisher: BioMed Central Ltd.
Document Type: Report
Length: 3,345 words
Lexile Measure: 1620L

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Abstract :

Objective In present study, the effects of the leaf extract of Pyrus biossieriana Buhse on tert-Butyl hydroperoxide (t-BHP) induced toxicity in the HepG2 cell line were investigated. Results HepG2 cells were exposed to different concentrations of both extract (1.5, 2.0, and 2.5 mg/mL) and t-BHP (100, 150, and 200 [mu]M). The total flavonoid and phenolic contents, the cell viability, lipid peroxidation, NO generation, and the total antioxidant capacity in cell media were assessed. The amount of arbutin was estimated 12.6% of the dry weight of leaves (equivalent to 126 mg/g). Additionally, the amounts of flavonoids and phenols in extract were estimated 119 mg/g and 418 mg/g, respectively. The cells incubated with t-BHP showed a significant decrease in survival (p Keywords: Antioxidant, Cytotoxicity, HepG2 cell line, Pyrus biossieriana Buhse, tert-Butyl hydroperoxide

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Gale Document Number: GALE|A675240744