The effect of new particle formation (NPF) on cloud condensation nuclei (CCN) varies widely in diverse environments. CCN or cloud droplets from NPF sources remain highly uncertain in the urban atmosphere; they are greatly affected by the high background aerosols and frequent local emissions. In this study, we quantified the effect of NPF on cloud droplet number concentration (CDNC, or N.sub.d) at typical updraft velocities (V) in clouds based on field observations on 25 May-18 June 2017 in urban Beijing. We show that NPF increases the N.sub.d by 32 %-40 % at V=0.3-3 m s.sup.-1 during the studied period. The N.sub.d is reduced by 11.8 Â± 5.0 % at V=3 m s.sup.-1 and 19.0 Â± 4.5 % at V=0.3 m s.sup.-1 compared to that calculated from constant supersaturations due to the water vapor competition effect, which suppresses the cloud droplet formation by decreasing the environmental maximum supersaturation (S.sub.max). The effect of water vapor competition becomes smaller at larger V that can provide more sufficient water vapor. However, under extremely high aerosol particle number concentrations, the effect of water vapor competition becomes more pronounced. As a result, although a larger increase of CCN-sized particles by NPF events is derived on clean NPF days when the number concentration of preexisting background aerosol particles is very low, no large discrepancy is presented in the enhancement of N.sub.d by NPF between clean and polluted NPF days. We finally reveal a considerable impact of the primary sources on the evaluation of the contribution of NPF to CCN number concentration (N.sub.CCN) and N.sub.d based on a case study. Our study highlights the importance of full consideration of both the environmental meteorological conditions and multiple sources (i.e., secondary and primary) to evaluate the effect of NPF on clouds and the associated climate effects in polluted regions.