Assessing Indoor Dust Interference with Human Nuclear Hormone Receptors in Cell-Based Luciferase Reporter Assays.

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From: Environmental Health Perspectives(Vol. 129, Issue 4)
Publisher: National Institute of Environmental Health Sciences
Document Type: Report
Length: 12,036 words
Lexile Measure: 1430L

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Abstract :

Background: Per- and polyfluoroalkyl substances (PFAS), organophosphate esters (OPEs), and polybrominated diphenyl ethers (PBDEs) are hormone-disrupting chemicals that migrate from building materials into air and dust. Objectives: We aimed to quantify the hormonal activities of 46 dust samples and identify chemicals driving the observed activities. Methods: We evaluated associations between hormonal activities of extracted dust in five cell-based luciferase reporter assays and dust concentrations of 42 measured PFAS, OPEs, and PBDEs, transformed as either raw or potency-weighted concentrations based on Tox21 high- throughput screening data. Results: All dust samples were hormonally active, showing antagonistic activity toward peroxisome proliferator- activated receptor (PPAR[gamma]2) (100%; 46 of 46 samples), thyroid hormone receptor (TR[beta]) (89%; 41 samples), and androgen receptor (AR) (87%; 40 samples); agonist activity on estrogen receptor (ER[alpha]) (96%; 44 samples); and binding competition with thyroxine ([T.sub.4]) on serum transporter transthyretin (TTR) (98%; 45 samples). Effects were observed with as little as 4 [micro]g of extracted dust. In regression models for each chemical class, interquartile range increases in potency-weighted or unknown-potency chemical concentrations were associated with higher hormonal activities of dust extracts (potency- weighted: ZPFAS-TR[beta], [up arrow] 28%, p

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Gale Document Number: GALE|A661609630