Noradrenaline regulates substance P release from rat dorsal root ganglion neurons in vitro

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Date: Oct. 2011
From: Neuroscience Bulletin(Vol. 27, Issue 5)
Publisher: Springer
Document Type: Article
Length: 278 words

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Byline: Yan-Jie Wang (1), Xing-Fu Li (1), Feng Ding (1), Qiang Shu (1), Li-Jun Song (1), Xiao Yu (1), Hua-Xiang Liu (1) Keywords: noradrenaline; substance P; dorsal root ganglion; a c2e3/4a,eoc'; Pc(c)e'"; eae 1cY=c e Abstract: Objective To determine whether activation and/or inhibition of [alpha]-adrenoreceptors influences substance P (SP) release from dorsal root ganglion (DRG) primary sensory neurons in vitro. Methods DRGs were dissected from 15-day embryonic Wistar rats. DRG neurons were dissociated and cultured for 2 d and then exposed to noradrenaline (NA) alone (1x10.sup.-4 mol/L), or along with the [alpha]1-adrenoreceptor antagonist prazosin (1x10.sup.-6 mol/L) or the [alpha]2-adrenoreceptor antagonist yohimbine (1x10.sup.-5 mol/L) for 4 d. Then, RT-PCR was used to determine the levels of preprotachykinin (PPT) mRNA encoding for SP and Western blot to assess the protein levels of SP. Basal and capsaicin (CAP)-evoked SP release were measured by enzyme-linked immunosorbent assay (ELISA). Results CAP-evoked SP release was sensitized by NA and this effect was inhibited by pre-incubation with prazosin but not with yohimbine. The levels of PPT mRNA, SP peptide, and basal SP release did not change significantly in any of the experimental conditions. Conclusion NA may significantly increase CAP-evoked SP release through activation of [alpha]-adrenoreceptors, which may contribute to noradrenergic pain modulation. c(r)c eSSa-ae?ae' aeaeaP[alpha]-e3/4a,eoc' aa1/2ae-a|a1/2+-aa1/2a$?a1a ceae 1cY=c e(dorsal root ganglion, DRG)cY=c a Pc(c)e'"(substance P, SP)ceae3/4a ae1ae3 ee3/415a$?(c)cWistara$?SSe1/4 DRGcY=c aa1a 2a$?(c)a, aa c2e3/4a,eoc' (noradrenaline, NA)(1x10.sup.-4 mol/L)a [alpha].sub.1-aa1/2ae(r)aeaaaaa(1x10.sup.-6 mol/L)+NA(1x10.sup.-4 mol/L)a [alpha].sub.2-aa1/2ae(r)aeae2ao"a(r)3/4(1x10.sup.-5 mol/ L)+NA(1x10.sup.-4 mol/L)aue24a$?(c)a c"RT-PCRae3aePSaeuDRGcY=c ac1/4c SPec1/2cPPT mRNAe!"e3/43/4aedeg'a13, c"Western blot ae3aePSaeuDRGcY=c aSPec1/2ce!"e3/43/4aedeg'a13, c"ePeac c ae NAac!aue2ae3/4eaC/a aoDRGcY=c ae3/4PSae$?c' aoae?acSPeae3/4e, [alpha].sub.1-aa1/2ae(r)aeaaaaaeC/a$?ca-e aeNAcaeao, e[alpha].sub.2-aa1/2ae(r)aeae2ao"a(r)3/4a,aoSScae$?a1/2c"a a"acSSa(r)eaae!a Pa,, PPT mRNAaedeg'a13a SPec1/2e!"e3/43/4aedeg'a13aSPcaoc!eae3/4eae2!aeae3/4eaeSSa*(r)a1/4a c e(r)o NAee?ae?ae' [alpha].sub.1-aa1/2aC/a aoDRGcY=c ae3/4PSae$?c' aoae?acSPeae3/4e, e?a,a1/2c"a-e1/2a,a c2e3/4a,eoc' e1/2cc1/4cedegeaea3a Author Affiliation: (1) Department of Rheumatology, Shandong University Qilu Hospital, Jinan, 250012, China Article History: Registration Date: 27/09/2011 Received Date: 30/06/2011 Accepted Date: 13/07/2011 Online Date: 29/09/2011

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Gale Document Number: GALE|A268314648