Effect of acidic pH on the invasion efficiency and the type III secretion system of Burkholderia thailandensis

Citation metadata

From: The Journal of Microbiology(Vol. 48, Issue 4)
Publisher: Springer
Document Type: Report
Length: 253 words

Document controls

Main content

Abstract :

Byline: Siroj Jitprasutwit (1), Wisansanee Thaewpia (2), Veerachat Muangsombut (1), Aroonlug Lulitanond (3), Chanvit Leelayuwat (3), Ganjana Lertmemongkolchai (3), Sunee Korbsrisate (1) Keywords: B. thailandensis; acidic pH; type III secretion system; invasion Abstract: Burkholderia thailandensis is a close relative of Burkholderia pseudomallei. These organisms are very similar, but B. thailandensis is far less virulent than B. pseudomallei. Nucleotide sequencing and analysis of 14 B. thailandensis isolates revealed variation in the regions coding for the type III secreted BipD protein. The degree of B. thailandensis BipD sequence variation was greater than that found in B. pseudomallei. Western blot analysis indicated that, unlike B. pseudomallei, B. thailandensis type III secreted proteins including BipD and BopE could not be detected in the supernatant of culture medium unless induced by acidic conditions. In addition, culturing B. thailandensis under acidic growth conditions (pH 4.5) can induce the ability of this bacterium to invade human respiratory epithelial cells A549. The identification of an environmental stimulus that increases the invasion capability of B. thailandensis invasion is of value for those who would like to use this bacterium as a model to study B. pseudomallei virulence. Author Affiliation: (1) Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand (2) Khon Kaen Regional Hospital, Ministry of Public Health, Khon Kaen, 40000, Thailand (3) Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand Article History: Registration Date: 18/08/2010 Received Date: 25/02/2010 Accepted Date: 11/07/2010 Online Date: 20/08/2010

Source Citation

Source Citation   

Gale Document Number: GALE|A235725375