Biosynthesis of Translation Inhibitor Klebsazolicin Proceeds through Heterocyclization and N-Terminal Amidine Formation Catalyzed by a Single YcaO Enzyme

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From: Journal of the American Chemical Society(Vol. 140, Issue 16)
Publisher: American Chemical Society
Document Type: Report
Length: 72 words

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Abstract :

Klebsazolicin (KLB) contains an N-terminal amidine ring and four azole heterocycles installed into a ribosomally synthesized precursor by dedicated maturation machinery. Using an in vitro system for KLB production, it is shown that the YcaO-domain KlpD maturation enzyme is a bifunctional cyclodehydratase required for the formation of both the core heterocycles and the N-terminal amidine ring. The versatility of KlpD opens up possibilities for rational introduction of modifications into various peptide backbones.

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Gale Document Number: GALE|A541807259