Lactase phlorizin hydrolase synthesis is decreased in protein-malnourished pigs

Citation metadata

From: The Journal of Nutrition(Vol. 127, Issue 5)
Publisher: American Institute of Nutrition
Document Type: Article
Length: 271 words

Document controls

Main content

Abstract :

We have examined the effect of protein malnutrition on brush border (BB) lactase phlorizin hydrolase (LPH) synthesis in young pigs. Two groups of four 3-wk-old pigs were fed diets containing either 19 g soy protein, 63 g carbohydrate and 5 g fat per 100 g diet (a protein-sufficient diet) or 3 g soy protein, 85 g carbohydrate and 5 g fat per 100 g diet (a protein-deficient diet). After 8 wk of consuming the diets, pigs were infused intravenously with 2[H.sub.3]-leucine for 8 h, then killed. The jejunum was collected for measurement of lactase activity, LPH mRNA abundance and the rate of LPH post-translational synthesis. Lactase activities did not differ between groups (mean 8.1 [+ or -] 1.2 [micro]mol [center dot] [min.sup.-1] [center dot] g [mucosa.sup.-1]). LPH mRNA abundance relative to elongation factor-1[Alpha] mRNA (the constitutive/reference mRNA) was significantly (P KEY WORDS: * intestinal brush border * lactase phlorizin hydrolase * protein malnutrition * mRNA * pigs

Source Citation

Source Citation   

Gale Document Number: GALE|A19511727