The cyclic AMP receptor protein (CRP) controls expression of the ferric uptake regulator (Fur) in Yersinia pestis.

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Date: July 2022
From: Canadian Journal of Microbiology(Vol. 68, Issue 7)
Publisher: NRC Research Press
Document Type: Report
Length: 4,121 words
Lexile Measure: 1330L

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Abstract :

Yersinia pestis, the causative agent of plague, is one of the most dangerous pathogens in the world. Both the cyclic AMP receptor protein (CRP) and ferric uptake regulator (Fur) are global regulators that control the expression of a great deal of genes involved in a variety of cellular functions in Y. pestis. In this work, two CRP box-like deoxyribonucleic acid (DNA) sequences were detected in the upstream DNA region offur, suggesting that the transcription offur might be directly regulated by CRP in Y. pestis. Thus, transcriptional regulation of fur by CRP was investigated by primer extension, quantitative real-time PCR, LacZ fusion, and electrophoretic mobility shift assays. The results demonstrated that CRP was able to bind the regulatory DNA region of fur to activate its transcription. The data presented here not only suggested that the CRP and Fur regulons were bridged together via the direct regulation of fur by CRP, but also provided us a deeper understanding of the transcriptional regulation of fur in Y. pestis. Keywords: Yersinia pestis, CRP, Fur, regulation Yersinia pestis, l'agent causal de la peste, est l'un des agents pathogenes les plus dangereux au monde. La proteine receptrice de l'AMPc (CRP) et le regulateur de l'absorption du fer (Fur) sont des regulateurs generaux qui controlent l'expression d'un grand nombre de genes impliques dans une variete de fonctions cellulaires chez Y. pestis. Dans ce travail, deux sequences d'ADN de type boite CRP ont ete detectees dans la region d'ADN en amont de fur, ce qui suggere que la transcription de fur pourrait etre directement regulee par la CRP chez Y. pestis. Ainsi, la regulation transcriptionnelle de fur par la CRP a ete etudiee par des essais d'extension d'amorce, PCR quantitative en temps reel, fusion LacZ et retard sur gel. Les resultats ont demontre que la CRP etait capable de se lier a la region d'ADN regulatrice de fur pour activer sa transcription. Les donnees presentees ici suggerent non seulement que les regulons CRP et Fur sont lies par la regulation directe de fur par la CRP, mais elles nous permettent egalement de mieux comprendre la regulation transcriptionnelle de fur chez Y. pestis. [Traduit par la Redaction] Mots-cles : Yersinia pestis, CRP, Fur, regulation

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Gale Document Number: GALE|A712300619