The Effect of Melanocyte Stimulating Hormone and Hydroxyapatite on Osteogenesis in Pulp Stem Cells of Human Teeth Transferred into Polyester Scaffolds

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From: Fibers and Polymers(Vol. 19, Issue 11)
Publisher: Springer
Document Type: Report
Length: 434 words

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Byline: Marziyeh Aghazadeh (1), Mohammad Samiei (2), Vahideh Raeisdasteh Hokmabad (3), Effat Alizadeh (4), Neda Jabbari (5), Alexander Seifalian (6), Roya Salehi (7) Keywords: Melanocyte stimulating hormone; Human dental pulp stem cells; Polyester scaffolds; Hydroxyapatite; Tissue engineering Abstract: Presently, tissue engineering is employed in the restoration and repair of tissue defects. Degradable scaffolds, stem cells and stimulating factors are employed in this method. In this study, the effect of melanocyte-stimulating hormone (MSH) and/or hydroxyapatite (HA) on proliferation, osteoblast differentiation, and mineralization of human dental pulp stem cells (hDPSCs) seeded on PLLA-PCL nanofibrous scaffolds was evaluated. For this aim, PLLA-PCL-HA nanofibrous scaffolds were fabricated using electrospinning method. FE-SEM images exhibited that all nanofibers had bead-free morphologies with average diameters ranging from 150--205 nm. Human DPSCs seeded into PLLA-PCL nanofibers were treated with MSH. Cell viability, proliferation, morphology, osteogenic potential, and the expression of tissue-specific genes were assessed by means of MTT assay, FE-SEM, alizarin red S staining, and RT-PCR analysis. hDPSCs exhibited improved adhesion and proliferation capacity on the PLLA-PCL-HA nanofibers treated with MSH compared to other groups (p Author Affiliation: (1) 0000 0001 2174 8913, grid.412888.f, Stem Cell Research Center and Oral Medicine Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran (2) 0000 0001 2174 8913, grid.412888.f, Endodontics Department of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran (3) 0000 0004 0382 4160, grid.412673.5, Department of Chemistry, University of Zanjan, Zanjan, 45195 313, Iran (4) 0000 0001 2174 8913, grid.412888.f, Stem Cell Research Center and Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran (5) 0000 0001 2174 8913, grid.412888.f, Under Graduate Student of Dental Faculty, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran (6) Nanotechnology and Regenerative Medicine Commercialisation Centre (NanoRegMed Ltd) The London BioScience Innovation Centre, London, NW1 0NH, United Kingdom (7) 0000 0001 2174 8913, grid.412888.f, Drug Applied Research Center and Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, 5166614711, Iran Article History: Registration Date: 23/11/2018 Received Date: 27/04/2018 Accepted Date: 23/07/2018 Online Date: 26/11/2018

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Gale Document Number: GALE|A563475658