Vasopressin regulates the renin-angiotensin-aldosterone system via V1a receptors in macula densa cells

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Publisher: American Physiological Society
Document Type: Author abstract
Length: 265 words

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Abstract :

The neuropeptide hormone arginine-vasopressin (AVP) is well known to exert its antidiuretic effect via the vasopressin V2 receptor (V2R), whereas the role of the vasopressin Vla receptor (V1aR) in the kidney remains to be clarified. Previously, we reported decreased plasma volume and blood pressure in V1a receptor-deficient ([V1aR.sup.-/-]) mice (Koshimizu T, Nasa Y, Tanoue A, Oikawa R, Kawahara Y, Kiyono Y, Adachi T, Tanaka T, Kuwaki T, Mod T. Proc Natl Acad Sci USA 103: 7807-7812, 2006). In this study, we investigated the role of V 1 aR in urine concentration, renal function, and the renin-angiotensin system (RAS) using [V1aR.sup.-/-] mice. Urine volume of [V1aR.sup.-/-] mice was greater than that of wild-type mice, particularly when water was loaded, while the glomerular filtration rate (GFR), urinary NaCl excretion, AVP-dependent cAMP generation, V2R, and aquaporin 2 (AQP2) expression in the kidney were lower, indicating that the diminished GFR and V2R-AQP2 system led to impaired urinary concentration in [V1aR.sup.-/-] mice. Since the GFR and V2R-AQP2 system are regulated by RAS, we analyzed renin and angiotensin II in [V1aR.sup.-/-] mice and found that the plasma renin and angiotensin II were decreased. The expression of renin in granule cells was decreased in [V1aR.sup.-/-] mice, which led to a decreased level of plasma renin. In addition, the expression of renin stimulators such as neuronal nitric oxide synthase and cyclooxygenase-2 in macula densa (MD) cells, where V1aR was specifically expressed, was decreased in [V1aR.sup.-/-] mice. These data indicate that AVP regulates body fluid homeostasis and GFR via the V1aR in MD cells by activating RAS and subsequently the V2R-AQP2 system. renin-angiotensin system

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Gale Document Number: GALE|A181896336