Enhanced KRT13 gene expression bestows radiation resistance in squamous cell carcinoma cells.

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Publisher: Springer
Document Type: Report; Brief article
Length: 288 words

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Keywords: KRT13; Radiation-resistant carcinoma cell strains; Serum-free defined culture Abstract Cancer metastasis and recurrence are potentially lethal. A small number of cancer cell groups called cancer stem cells (CSCs) have both stem cell capacity and cancer-forming ability and are reported to play important roles in cancer metastasis and recurrence. These CSCs are considered to be radiation-resistant (RR). Therefore, understanding the biological effects of radiation on squamous cell carcinoma (SCC) cell lines in vitro and in vivo might be worthwhile to circumvent radiation resistance. Currently, there are no reports on the establishment of RR-SCC cells in serum-free defined culture, which mimics biological mechanisms and prevents instability of using serum in the culture medium. We isolated radiation-resistant strains, designated A431-LDR and A431-HDR, from A431 cells derived from vulval SCC and irradiated them with a total dose of 60 Gy at a low-dose rate (2.2 Gy/d) (RM1000) and a high-dose rate (5 Gy/5.75min) in serum-free defined culture. These cells exhibited high sphere-forming and migration ability in vitro and high tumor-forming ability in nude mice xenografts. Overexpression of KRT13 in A431-RR cells might play a role in its radiation-resistant characteristics. These cells might be useful not only to study cancer stem cells but also to study the circumvention of radiation resistance by novel cancer treatment modalities. Author Affiliation: (1) Department of Molecular Oral Medicine and Maxillofacial Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734-8553, Hiroshima, Japan (2) National Hospital of Odonto Stomatology, Ho Chi Minh City, Vietnam (3) Oral and Maxillofacial Surgery, Hiroshima University Hospital, Hiroshima, Japan (4) School of Medical Sciences, University of East Asia, Shimonoseki 751-8503, Japan (h) tetsuok@hiroshima-u.ac.jp Article History: Registration Date: 12/18/2020 Received Date: 11/23/2020 Accepted Date: 12/18/2020 Online Date: 02/03/2021 Byline:

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Gale Document Number: GALE|A656670599