Functional and Evolutionary Significance of Human MicroRNA Seed Region Mutations

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From: PLoS ONE(Vol. 9, Issue 12)
Publisher: Public Library of Science
Document Type: Article
Length: 4,416 words
Lexile Measure: 1620L

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Abstract :

MicroRNAs have emerged in recent years as important regulators of cell function in both normal and diseased cells. MiRNAs coordinately regulate large suites of target genes by mRNA degradation and/or translational inhibition. The mRNA target specificities of miRNAs in animals are primarily encoded within a 7 nt "seed region" mapping to positions 2-8 at the molecule's 5' end. We here combine computational analyses with experimental studies to explore the functional significance of sequence variation within the seed region of human miRNAs. The results indicate that a substitution of even a single nucleotide within the seed region changes the spectrum of mRNA targets by 50%. The high functional cost of even single nucleotide changes within seed regions is consistent with their high sequence conservation among miRNA families both within and between species and suggests processes that may underlie the evolution of miRNA regulatory control.

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Gale Document Number: GALE|A418634925