Induction of integration-free human-induced pluripotent stem cells under serum- and feeder-free conditions.

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Publisher: Springer
Document Type: Report; Brief article
Length: 301 words

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Keywords: Human-induced pluripotent stem cells; Peripheral blood mononuclear cells; Reprogramming efficiency; hESF9 serum-free defined media Abstract Human-induced pluripotent stem cells (hiPSCs) have shown great potential toward practical and scientific applications. We previously reported the generation of human dental pulp stem cells using non-integrating replication-defective Sendai virus (SeVdp) vector in feeder-free culture with serum-free medium hESF9. This study describes the generation of hiPSCs from peripheral blood mononuclear cells to increase the donor population, while reducing biopsy invasiveness. From 6-d-old primary culture of peripheral blood mononuclear cells (PBMCs) with IL-2, hiPSCs were established using SeVdp(KOSM)302L with recombinant Laminin-511 E8 fragments under serum-free condition. The established PBMC-derived hiPSCs showed pluripotency and differentiation ability both in vivo and in vitro. In addition, we evaluated microarray data from PBMC- and dental pulp--derived hiPSCs. These hiPSCs will be beneficial for characterizing the molecular mechanisms of cellular differentiation and may provide useful substrates for developing cellular therapeutics. Author Affiliation: (1) Department of Oral and Maxillofacial Surgery, Hiroshima University Hospital, 1-2-3, Kasumi, Minami-ku, 734-8553, Hiroshima-City, Japan (2) Department of Oral Maxillofacial Surgery, Hiroshima Red Cross Hospital & Atomic-bomb Survivors Hospital, Hiroshima, Japan (3) TOKIWA-Bio, Inc., Tsukuba, Ibaraki, Japan (4) Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan (5) Laboratory of Gene Regulation, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan (6) Department of Molecular Oral Medicine and Maxillofacial Surgery, Division of Applied Life Science, Graduate Institute of Biomedical and Health Science, Hiroshima University, Hiroshima, Japan (j) tetsuok@hiroshima-u.ac.jp Article History: Registration Date: 10/15/2019 Received Date: 08/06/2019 Accepted Date: 10/15/2019 Online Date: 11/25/2019 Byline: Atsuko Hamada (1), Eri Akagi (2), Sachiko Yamasaki (1), Hirotaka Nakatao (1), Fumitaka Obayashi (1), Manami Ohtaka (3, 4), Ken Nishimura (5), Mahito Nakanishi (4, 3), Shigeaki Toratani (6), Tetsuji Okamoto (corresponding author) (1, 6, j)

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Gale Document Number: GALE|A637834758