Discovery of Intermediates of lacZ beta-Galactosidase Catalyzed Hydrolysis Using dDNP NMR

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Date: Feb. 28, 2018
From: Journal of the American Chemical Society(Vol. 140, Issue 8)
Publisher: American Chemical Society
Document Type: Report
Length: 52 words

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Abstract :

The enzyme lacZ beta-galactosidase from Escherichia coli is subjected to hyperpolarized substrate, and previously unknown reaction intermediates are observed, including a 1,1-linked disaccharide. The enzyme is known for making 1,6-transglycosylation, producing products like allolactose, which are also substrates. The findings suggest that hydrolysis and transglycosylation might be more complex than previously described.

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Gale Document Number: GALE|A536740060