Chemotactic response of Vibrio coralliilyticus to mucus from various coral species.

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From: Canadian Journal of Microbiology(Vol. 67, Issue 7)
Publisher: NRC Research Press
Document Type: Report
Length: 3,168 words
Lexile Measure: 1370L

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Abstract :

Vibrio coralliilyticus, a prominent pathogenic bacteria, is known to cause tissue damage in the coral Pocillopora damicornis and is attracted towards the coral via chemotaxis. However, the potential of V. coralliilyticus to infect most of the other coral hosts via chemotaxis is unknown. In this study, we used capillary assays to quantify the chemotactic response of V. coralliilyticus to the mucus of four tank-cultivated coral species (Cataphyllia jardine, Mussidae sp., Nemenzophyllia turbida, and Euphyllia ancora), and mucus from three wild coral species (Acropora sp., Porites sp., and Montipora sp.). The bacteria showed a positive chemotactic response to each coral mucus tested, with the highest response recorded to the mucus of Acropora sp. and the lowest response to the mucus of Montipora sp. A microfluidic chip was then used to assess the chemotactic preference of V. coralliilyticus to the mucus of the tank cultivated corals. Here too, the bacterium showed positive response, but with a slightly different ranking order. The strong chemotactic response of V. coralliilyticus towards the mucus tested could indicate a broader host range of V. coralliilyticus, and by extension, indicate a threat to weakened coral reefs worldwide. Key words: chemotaxis, Vibrio coralliilyticus, coral hosts, microfluidics. Vibrio coralliilyticus, une bacterie pathogene importante, est connue pour provoquer des dommages tissulaires dans le corail Pocillopora damicornis et elle est attiree vers le corail par chimiotaxie. Toutefois, le potentiel de V. coralliilyticus a infecter la plupart des autres coraux hotes par chimiotaxie est inconnu. La presente etude a utilise des tests capillaires pour quantifier la reponse chimiotactique de V. coralliilyticus au mucus de quatre coraux cultives en bassin, Cataphyllia jardine, Mussidae sp., Nemenzophyllia turbida et Euphyllia ancora et au mucus de trois coraux sauvages, Acropora sp., Porites sp. et Montipora sp. Les bacteries montraient une reponse chimiotactique positive a chaque mucus de corail teste, la reponse la plus elevee etant enregistree au mucus d'Acropora sp. et la plus faible au mucus de Montipora sp. Une puce microfluidique a ensuite ete utilisee pour evaluer la preference chimiotactique de V. coralliilyticus au mucus des coraux cultives en bassin. La aussi, les bacteries presentaient une reponse positive avec un ordre de classement legerement different. La forte reponse chimiotactique de V. coralliilyticus envers les mucus testes pourrait indiquer une gamme d'hotes plus etendue de V. coralliilyticus et, par extension, sa menace pour les recifs coralliens affaiblis a travers le monde. [Traduit par la Redaction] Mots-cles : chimiotaxie, Vibrio coralliilyticus, hotes coralliens, microfluidique.

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Gale Document Number: GALE|A668735845