HMGA1 is a new target of miR-195 involving isoprenaline-induced cardiomyocyte hypertrophy

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From: Biochemistry (Moscow)(Vol. 79, Issue 6)
Publisher: Springer
Document Type: Report
Length: 2,646 words
Lexile Measure: 1350L

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Abstract :

Emerging data have shown that microRNAs (miRNAs) have important functions in the processes of cardiac hypertrophy and heart failure that occur during the postnatal period. Cardiac overexpression of miR-195 results in pathological cardiac growth and heart failure in transgenic mice. In the present study, we analyzed the roles of miR- 195 in cardiomyocyte hypertrophy and found that miR-195 was greatly upregulated during isoprenaline-induced cardiomyocyte hypertrophy. By using mRNA microarray and molecular approach, we identified a novel putative target of miR-195 called high-mobility group A1 (HMGA1). Total mRNA microarray showed that HMGA1 was downregulated in primary cardiomyocytes that overexpressed miR-195. Using luciferase activity assay, we demonstrated that miR-195 interacts with the 3'-untranslated region of HMGA1 mRNA. Moreover, we showed that miR-195 in primary cardiomyocytes downregulates the expression of HMGA1 at the protein level. Taken together, our data demonstrated that miR- 195 can negatively regulate a new target, HMGA1, which is involved in cardiomyocyte hypertrophy. DOI: 10.1134/S0006297914060078 Key words: microRNA-195, cardiomyocyte hypertrophy, microRNA target, HMGA1

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Gale Document Number: GALE|A379570340