Distinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae.

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From: BMC Research Notes(Vol. 14, Issue 1)
Publisher: BioMed Central Ltd.
Document Type: Report
Length: 2,975 words
Lexile Measure: 1380L

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Abstract :

Objective The current experiment aimed to assess the impact of detergents such as 3% Triton X-100, 1% peracetic acid, 1% Tween-20, and 1% SDS in combination with Trypsin-EDTA on acellularization of ovine aortae after 7 days. Results Hematoxylin-Eosin staining showed an appropriate acellularization rate in ovine aortae, indicated by a lack of cell nuclei in the tunica media layer. DAPI staining confirmed the lack of nuclei in the vascular wall after being exposed to the combination of chemical and enzymatic solutions. Verhoeff-Van Gieson staining showed that elastin fibers were diminished in acellular samples compared to the control group while collagen stands were unchanged. CCK-8 survival assay showed enhanced viability in human umbilical vein endothelial cells 5 days after being cultured on decellularized samples compared to the cells cultured on a plastic surface (p Keywords: Ovine aorta, Acellularization, Endothelial cells, Survival rate, Attachment

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Gale Document Number: GALE|A661377886