Bioluminescence imaging of Arc expression enables detection of activity-dependent and plastic changes in the visual cortex of adult mice

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From: Brain Structure and Function(Vol. 216, Issue 2)
Publisher: Springer
Document Type: Report
Length: 286 words

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Byline: Hironori Izumi (1), Tetsuya Ishimoto (1), Hiroshi Yamamoto (2), Hisao Nishijo (3), Hisashi Mori (1) Keywords: Arc; Neuronal-activity-dependent gene expression; Bioluminescence; BAC transgenic mouse; Visual cortex; Plasticity Abstract: Induction of the activity-regulated cytoskeleton-associated protein gene (Arc), one of the immediate early genes, in the brain correlates with various sensory processes, natural behaviors, and pathological conditions. Arc is also involved in synaptic plasticity during development. Thus, in vivo monitoring of Arc expression is useful for the analysis of physiological and pathological conditions in the brain. Recently, in vivo imaging of Arc expression using various green fluorescent protein-based probes has been reported however, these probes can only be applied for the detection of fluorescence signals from superficial layers of the cortex with some autofluorescence noise. Here, we generated a novel transgenic mouse strain to monitor the neuronal-activity-dependent Arc expression using bioluminescence signals in vivo. Because of the very high sensitivity with a high signal-to-noise ratio, we detected neuronal-activity-dependent plastic changes in the bioluminescence signal intensity in the mouse visual cortex after visual deprivation, suggesting structural plasticity after peripheral lesions in adults. We also detected drastic changes in bioluminescence signals after seizure induction with kainic acid. Our novel mouse strain will be valuable for the continuous monitoring of neuronal-activity-dependent Arc expression in the brain under physiological and pathological conditions. Author Affiliation: (1) Department of Molecular Neuroscience, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, 930-0194, Japan (2) Division of Animal Experimental Laboratory, Life Science Research Center, University of Toyama, Toyama, 930-0194, Japan (3) Department of System Emotional Science, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, 930-0194, Japan Article History: Registration Date: 06/12/2010 Received Date: 13/10/2010 Accepted Date: 06/12/2010 Online Date: 19/12/2010

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Gale Document Number: GALE|A256780294