Controlled Growth of Chinese Hamster Ovary Cells and High Expression of Antibody-IL-2 Fusion Proteins by Temperature Manipulation

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Date: Dec. 2005
From: Biotechnology Letters(Vol. 27, Issue 23-24)
Publisher: Springer
Document Type: Author abstract
Length: 162 words

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Abstract :

Byline: Ming Shi (1), Zhigang Xie (1), Ming Yu (1), Beifen Shen (1), Ning Guo (1) Keywords: antibody; cell cycle; fusion protein; gene expression; Interleukin 2; low temperature Abstract: Growth and the expression of the anti-ErbB2 scFv-Fc-IL2 fusion protein in Chinese hamster ovary (CHO) cells were in association at 37 degC. The expression of the fusion protein was no more than 25 ug/ml. At 30 degC the cell growth was arrested but the cells continued to produce the fusion protein up to 60--80 ug/ml. About 50% of CHO cells were rapidly blocked in G2/M phase after the temperature was shifted from 37 to 30 degC. Lowering temperature resulted in cell growth arrest, but maintained cell viability for a longer time and enhanced the production of the antibody-IL-2 fusion protein in CHO cells. Author Affiliation: (1) Department of Molecular Immunology, Institute of Basic Medical Sciences, 27 Taiping Road, 100850, Beijing, P.R. China Article History: Registration Date: 10/10/2005 Received Date: 08/07/2005 Accepted Date: 27/09/2005

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Gale Document Number: GALE|A162521903