In vitro transduction of antimicrobial resistance genes into Escherichia coli isolates from backyard poultry in Mexico.

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From: Canadian Journal of Microbiology(Vol. 67, Issue 5)
Publisher: NRC Research Press
Document Type: Report
Length: 7,130 words
Lexile Measure: 1540L

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Abstract :

The transmission of multidrug-resistant pathogens and antimicrobial resistance genes is an emerging problem involving multiple factors (humans, domestic animals, wildlife). The aim of this study was to investigate the presence of Escherichia coli isolates with different antimicrobial resistance genes from backyard poultry and to demonstrate the in vitro transduction phenomenon of these genes between phages from migratory wild birds and poultry E. coli isolates. We collected 197 E. coli isolates from chickens, turkeys, and ducks in backyard production units (northern region of the State of Mexico). Isolates were resistant to ampicillin (80.7%), tetracycline (64.4%), carbenicillin (56.3%), and nalidixic acid and trimethoprim-sulfamethoxazole (both, 26.9%). Moreover, the genes [bla.sub.TEM] (56.3%), tetB (20.8%), tetA (19.2%), sulI (7.6%), sulII (10.1%), qnrA (9.6%), and qnrB (5.5%) were found. In vitro transduction using phages from migratory wild birds sampled in the wetland Chimaliapan (State of Mexico) was successfully achieved. It was possible to transduce qnrA, tetB, [bla.sub.TEM], and sulII genes to E. coli isolates from poultry. This is the first report that describes the transduction of antimicrobial resistance genes from phages of migratory wild birds to poultry and suggests the possible transmission in backyard production units. Key words: backyard poultry, antimicrobial resistance, bacteriophages, transduction, wild birds. La transmission d'agents pathogenes multiresistants et de genes de resistance aux antimicrobiens est un probleme en emergence impliquant de multiples facteurs (humains, animaux domestiques, faune). L'objectif de cette etude etait d'etudier la presence d'isolats d'Escherichia coli comportant differents genes de resistance aux antimicrobiens provenant de volailles urbaines et de demontrer le phenomene de transduction in vitro de ces genes entre les phages d'oiseaux sauvages migrateurs et les isolats d'E. coli de volaille. Les auteurs ont recolte 197 isolats d'E. coli de poulets, de dindes et de canards dans des unites de production urbaines (region nord de l'Etat de Mexico). Les isolats etaient resistants a l'ampicilline (80,7 %), a la tetracycline (64,4 %), a la carbenicilline (56,3 %), ainsi qu'a l'acide nalidixique et au trimethoprime-sulfamethoxazole (26,9 % chacun). De plus, les genes [bla.sub.TEM] (56,3 %), tetB (20,8 %), tetA (19,2 %), sulI (7,6 %), sulII (10,1 %), qnrA (9,6 %) et qnrB (5,5 %) ont ete trouves. La transduction in vitro a l'aide des phages d'oiseaux sauvages migrateurs echantillonnes dans la zone humide de Chimaliapan (Etat de Mexico) a ete realisee avec succes. Il a ete possible de transduire les genes qnrA, tetB, [bla.sub.TEM] et sulII a des isolats d'E. coli provenant de volailles. Il s'agit du premier rapport qui decrit la transduction des genes de resistance aux antimicrobiens des phages des oiseaux sauvages migrateurs aux volailles et suggere la transmission possible dans les unites de production urbaines. [Traduit par la Redaction] Mots-cles: volailles urbaines, resistance aux antimicrobiens, bacteriophages, transduction, oiseaux sauvages.

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Gale Document Number: GALE|A662459558