Antifungal activity of [[K.sup.3]]temporin-SHa against medically relevant yeasts and moulds.

Citation metadata

From: Canadian Journal of Microbiology(Vol. 68, Issue 6)
Publisher: NRC Research Press
Document Type: Report
Length: 5,252 words
Lexile Measure: 1300L

Document controls

Main content

Abstract :

Few antifungal agents are currently available for the treatment of fungal infections. Antimicrobial peptides (AMPs), which are natural molecules involved in the innate immune response of many organisms, represent a promising research method because of their broad killing activity. The aim of this study was to assess the activity of a frog AMP, [[K.sup.3]]temporin-SHa, against some species of yeasts and moulds, and to further explore its activity against Candida albicans. MIC determinations were performed according to EUCAST guidelines. Next, the activity of [[K.sup.3]]temporin-SHa against C. albicans was explored using time-killing curve experiments, membrane permeabilization assays, and electron microscopy. Finally, chequerboard assays were performed to evaluate the synergy between [[K.sup.3]]temporin-SHa and amphotericin B or fluconazole. [[K.sup.3]]temporin-SHa was found to be active in vitro against several yeasts with MIC between 5.5 and 45 [micro]M. [[K.sup.3]]temporin-SHa displayed rapid fungicidal activity against C. albicans (inoculum was divided into two in less than an hour and no viable colonies were recovered after 5 h) with a mechanism that could be due to membrane permeabilization. [[K.sup.3]]temporin-SHa was synergistic with amphotericin B against C. albicans (FICI = 0.303). [[K.sup.3]]temporin-SHa could represent an additional tool to treat several Candida species and C. neoformans. Key words: antimicrobial peptides, Candida, [[K.sup.3]]temporin-SHa, antifungal compound. Peu d'agents antifongiques sont disponibles pour combattre les infections fongiques. Les peptides antimicrobiens (PAM), qui sont des molecules naturelles faisant partie de la reponse immunitaire innee de nombreux organismes, representent une voie de recherche prometteuse en raison de leur large activite antimicrobienne. Le but de cette etude etait d'evaluer l'activite d'un PAM de grenouille, la temporine [[K.sup.3]]SHa, contre certaines especes de levures et de moisissures, et d'explorer davantage son activite contre Candida albicans. Les determinations des CMI ont ete effectuees conformement aux directives de l'EUCAST. Ensuite, l'activite de la temporine [[K.sup.3]]SHa contre C. albicans a ete etudiee par des experiences de courbe de mortalite, des tests de permeabilisation membranaire et de la microscopie electronique. Enfin, des tests en damier ont ete realises pour evaluer la synergie entre la temporine [[K.sup.3]]SHa et l'amphotericine B ou le fluconazole. La temporine [[K.sup.3]]SHa s'est averee active, in vitro, contre plusieurs levures avec des CMI comprises entre 5,5 et 45 [micro]M. La temporine [[K.sup.3]]SHa a montre une activite fongicide rapide contre C. albicans (l'inoculum a ete reduit par deux en moins d'une heure et aucune colonie viable n'a ete recuperee apres 5 heures) avec un mecanisme qui pourrait etre du a la permeabilisation de la membrane. Enfin, la temporine [[K.sup.3]]SHa a agi de maniere synergique avec l'amphotericine B contre C. albicans (FICI = 0,303). La temporine [[K.sup.3]]SHa pourrait constituer un outil supplementaire pour traiter plusieurs especes de Candida et C. neoformans. [Traduit par la Redaction] Mots-cles : peptides antimicrobiens, Candida, temporine [[K.sup.3]]SHa, compose antifongique.

Source Citation

Source Citation   

Gale Document Number: GALE|A706322310