Identification of plasmids from Brazilian Chromobacterium violaceum strains.

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From: Canadian Journal of Microbiology(Vol. 68, Issue 1)
Publisher: NRC Research Press
Document Type: Report
Length: 5,483 words
Lexile Measure: 1420L

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Abstract :

Chromobacterium violaceum is an opportunistic pathogen found in tropical and subtropical regions worldwide. Chromobacterium violaceum infections are difficult to treat, and many strains are resistant to antibiotics. Recently, a novel plasmid (pChV1) was discovered in the type strain ATCC 12472, suggesting that other C. violaceum strains may harbor extra-chromosomal DNA. The aim of the present study was to detect and compare new plasmids in Brazilian strains of C. violaceum using next-generation sequencing techniques. We obtained draft genomes of six plasmids from strains isolated from the Amazon region and aligned them with pChV1. At least three plasmids, CVAC05, CVACO2, and CVT8, were similar to pChV1. Phylogenetic analysis suggested that these new extra-chromosomal DNA sequences have a common origin with pChV1 but have diverged. Many of the ORFs detected were related to plasmid segregation/maintenance, viral structural proteins, and proteins with unknown functions. These findings may enable better genetic manipulation of C. violaceum, which will enhance our ability to exploit this valuable microorganism in industrial and clinical applications. Key words: plasmids, biotechnology, genomics, microbial phylogenetics, bacteriophages, antibiotic resistance. Chromobacterium violaceum est un organisme pathogene opportuniste present dans les regions tropicales et subtropicales a travers le monde. Les infections a C. violaceum sont difficiles a traiter, et de nombreuses souches sont resistantes aux antibiotiques. Recemment, un nouveau plasmide (pChV1) a ete decouvert dans la souche type ATCC 12472, suggerant que d'autres souches de C. violaceum peuvent heberger de l'ADN extrachromosomique. L'objectif de la presente etude etait de detecter et de comparer de nouveaux plasmides dans des souches bresiliennes de C. violaceum en utilisant des techniques de sequencage de nouvelle generation. Les auteurs ont obtenu les genomes preliminaires de six plasmides provenant de souches isolees de la region amazonienne et les ont alignes par rapport a pChV1. Au moins trois plasmides, CVAC05, CVACO2, et CVT8, etaient similaires a pChV1. L'analyse phylogenetique suggere que ces nouvelles sequences d'ADN extrachromosomique ont une origine commune avec pChV1, mais ont diverge. Un grand nombre de CLO detectes etaient lies a la segregation/maintien des plasmides, aux proteines structurelles virales et aux proteines aux fonctions inconnues. Ces resultats peuvent permettre une meilleure manipulation genetique de C. violaceum, ce qui ameliorera notre capacite a exploiter ce precieux microorganisme dans des applications industrielles et cliniques. [Traduit par la Redaction] Mots-cles : plasmides, biotechnologie, genomique, phylogenetique microbienne, bacteriophages, resistance aux antibiotiques.

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Gale Document Number: GALE|A690284822