Diversity and function of microbial communities in the sand sheath of Agropyron cristatum by metagenomic analysis.

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Date: Mar. 2022
From: Canadian Journal of Microbiology(Vol. 68, Issue 3)
Publisher: NRC Research Press
Document Type: Report
Length: 6,620 words
Lexile Measure: 1490L

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Abstract :

The roots of most gramineous plants are surrounded by a variety of microorganisms; however, few studies have focused on the rhizosheath of psammophytes. Therefore, in this study, we used Illumina HiSeq high-throughput sequencing technology to analyse the composition and functional diversity of microbial communities in the rhizosheath of sand-grown Agropyron cristatum (L.) Gaertn. We found that the number of species and functions of microbial communities gradually decreased from the rhizosheath to the bulk soil. Thus, the microbial composition of the rhizosheath was richer and more diverse, and the abundance of bacteria, including Sphingosinicella, Rhizorhabdus, Friedmanniella, Geodermatophilus, Blastococcus, and Oscilatoria, was higher, and the abundance of fungi, such as Mycothermus, was higher. The abundance of C[O.sub.2] fixation-related genes (acsA, Pcc, and cbbL) in the carbon cycle; N[O.sub.3.sup.-], N[O.sub.2.sup.-], N[H.sub.2]OH, and [N.sub.2] transformation genes (nrtP, nirS, hao, and nifK) in the nitrogen cycle; soxB/A/C, Sat, and dsrB genes in the sulphur cycle; and 1-phosphate mannitol dehydrogenase (MtlD) gene and polyketide synthase gene (pks) were higher in the rhizosheath than in the bulk soil, as well as genes related to phosphorus uptake in the phosphorus cycle. Our findings showed that the rhizosheath may host the predominant microbial species related to the formation of a rhizosheath. Key words: Agropyron cristatum, rhizosheath, Illumina sequencing, community structure, functional diversity. Les racines de la plupart des graminees sont entourees d'une variete de microorganismes; cependant, peu d'etudes ont porte sur la rhizosphere des psammophytes. Consequemment, dans cette etude, les auteurs ont utilise la technologie de sequencage a haut debit Illumina HiSeq pour analyser la composition et la diversite fonctionnelle des communautes microbiennes dans la rhizosphere d'Agropyron cristatum (L.) Gaertn cultive dans le sable. Ils ont constate que le nombre d'especes et les fonctions des communautes microbiennes diminuaient progressivement de la rhizosphere au sol. Ainsi, la composition microbienne de la rhizosphere etait plus riche et plus diverse; en effet, l'abondance des bacteries, y compris Sphingosinicella, Rhizorhabdus, Friedmanniella, Geodermatophilus, Blastococcus, et Oscilatoria, etait plus elevee; de meme, l'abondance des champignons, tels que Mycothermus, etait plus elevee. L'abondance des genes lies a la fixation du C[O.sub.2] (acsA, Pcc, et cbbL) dans le cycle du carbone; les genes de transformation de N[O.sub.3.sup.-], N[O.sub.2.sup.-], N[H.sub.2]OH, et [N.sub.2] (nrtP, nirS, hao, et nifK) dans le cycle de l'azote; les genes soxB/A/C, Sat, dsrB dans le cycle du soufre; et le gene de la 1-phosphate mannitol deshydrogenase (MtlD) et le gene de la polyketide synthase (pks) etaient plus eleves dans la rhizosphere que dans le sol en vrac, ainsi que les genes lies a l'absorption du phosphore dans le cycle du phosphore. Ces resultats montrent que la peut heberger les especes microbiennes predominantes liees a la formation d'une rhizogaine. [Traduit par la Redaction] Mots-cles : Agropyron cristatum, rhizogaine, sequencage Illumina, structure de la communaute, diversite fonctionnelle.

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Gale Document Number: GALE|A696008265