Excitotoxicity effects of glutamate on human neuroblastoma SH-SY5Y cells via oxidative damage

Citation metadata

Date: Feb. 2010
From: Neuroscience Bulletin(Vol. 26, Issue 1)
Publisher: Springer
Document Type: Article
Length: 331 words

Document controls

Main content

Abstract :

Byline: Zhong-Wei Sun (1), Lan Zhang (1), Shu-Jia Zhu (1), Wen-Chun Chen (1), Bing Mei (1) Keywords: glutamate; excitotoxicity; cytosolic calcium; oxidative damage; edeg*aedeg"e,; a'aY=aeSSae-; eaeuec| a; aedegSSaaea1/4$? Abstract: Objective To investigate the mechanisms of excitotoxic effects of glutamate on human neuroblastoma SH-SY5Y cells. Methods SH-SY5Y cell viability was measured by MTT assay. Other damaged profile was detected by lactate dehydrogenase (LDH) release and by 4 , 6-diamidino-2-phenylindole (DAPI) staining. The cytosolic calcium concentration was tested by calcium influx assay. The glutamate-induced oxidative stress was analyzed by cytosolic glutathione assay, superoxide dismutase (SOD) assay and extracellular malondialdehyde (MDA) assay. Results Glutamate treatment caused damage in SHSY5Y cells, including the decrease of cell viability, the increase of LDH release and the alterations of morphological structures. Furthermore, the concentration of cytoplasmic calcium in SH-SY5Y cells was not changed within 20 min following glutamate treatment, while cytosolic calcium concentration significantly increased within 24 h after glutamate treatment, which could not be inhibited by MK801, an antagonist of NMDA receptors, or by LY341495, an antagonist of metabotropic glutamate receptors. On the other hand, oxidative damage was observed in SH-SY5Y cells treated with glutamate, including decreases in glutathione content and SOD activity, and elevation of MDA level, all of which could be alleviated by an antioxidant Tanshinone IIA (Tan IIA, a major active ingredient from a Chinese plant Salvia Miltiorrhiza Bge). Conclusion Glutamate exerts toxicity in human neuroblastoma SH-SY5Y cells possibly through oxidative damage, not through calcium homeostasis destruction mediated by NMDA receptors. c(r)c aeC/e(r)"edeg*aedeg"e,a-1/4e'aoocY=c ae-c ec$?c e(SH-SY5Y cells)a'aY=aeSSae-aea1/4$?caeoaPa ae1ae3 MTTae3aePSaeuSH-SY5Yc eaae' c aeua(r)a13e,e+-aedegC/ePeae3/4eeSSa-c eaea1/4$?c"ao| DAPIaee2ae3eSSa-c eaao!a1/2C/aea|c1c1 eaeuae3aePSaeueaeuec| aaeuao|aa a Y=eaedeg*e+-ce1/2a ePaedegSSac(c)aeSSaePae' aeSSaea$?a,aoea ecaedegSSaaoae?cPaea c ae edeg*aedeg"e,a-1/4e'SH-SY5Yc eaae, aae!aae' ca,ea a13e,e+-aedegC/ePeae3/4eaC/a$?aa1/2C/aec aeacae1a edeg*aedeg"e,a$?c20 min a, eaeuec| aaeuao|ae ae3/4eae1a, ea$?c24 h a, eaeuec| aa$?SSeaC/a , a,MK801 (NMDAaa1/2ae(r)aea)aLY341495 (a PSedegC/aedeg*aedeg"e,aa1/2ae(r)aea)aa,e1/2aeaPec| aaaeucaC/a$? edeg*aedeg"e,a-a-1/4e'SH-SY5YaedegSSaaea1/4$?, aae!eaedeg*e+-ce1/2a aeSSea1/2a ea$?ee'"e?aedegSSaaoSSc(c)a,aoeaedeg'a13ae e?aoaedegSSaaea1/4$?a c e(r)o edeg*aedeg"e,a-1/4e'SH-SY5Yc ea'aY=aeSSae-aea1/4$?a-e1/2ae-ee?aedegSSaaea1/4$?aoSScc, ea,a3/4euaoNMDA aa1/2a a-1/4cec"3aecc 'aa Author Affiliation: (1) Shanghai Institute of Brain Functional Genomics, and Key Laboratory of Brain Functional Genomics, MOE & STCSM, East China Normal University, Shanghai, 200062, China Article History: Registration Date: 02/02/2010 Received Date: 13/08/2009 Accepted Date: 28/10/2009 Online Date: 03/02/2010 Article note: These authors contributed equally to this work.

Source Citation

Source Citation   

Gale Document Number: GALE|A232782352