Insights into Carbon Metabolism Provided by Fluorescence In Situ Hybridization-Secondary Ion Mass Spectrometry Imaging of an Autotrophic, Nitrate-Reducing, Fe(II)-Oxidizing Enrichment Culture

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From: Applied and Environmental Microbiology(Vol. 84, Issue 9)
Publisher: American Society for Microbiology
Document Type: Article
Length: 88 words

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Abstract :

The article uses a combination of catalyzed amplification reporter deposition fluorescence in situ hybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (NanoSIMS) to analyze community dynamics, single-cell activities, and interactions among the two most abundant microbial community members under autotrophic and heterotrophic growth conditions. CARD-FISH cell counts show the dominance of the Fe(II) oxidizer Gallionellaceae sp. under autotrophic conditions as well as of Bradyrhizobium spp. under heterotrophic conditions. The study furthermore extends the understanding of the microbial reactions which interlink the nitrogen and Fe cycles in the environment.

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Gale Document Number: GALE|A542561917