Aeromonas punctata derived depolymerase improves susceptibility of Klebsiella pneumoniae biofilm to gentamicin

Citation metadata

Date: June 11, 2015
From: BMC Microbiology(Vol. 15, Issue 1)
Publisher: BioMed Central Ltd.
Document Type: Article
Length: 6,864 words
Lexile Measure: 1470L

Document controls

Main content

Abstract :

Background To overcome antibiotic resistance in biofilms, enzymes aimed at biofilm dispersal are under investigation. In the present study, applicability of an Aeromonas punctata derived depolymerase capable of degrading the capsular polysaccharide (CPS) of Klebsiella pneumoniae, in disrupting its biofilm and increasing gentamicin efficacy against biofilm was investigated. Results Intact biofilm of K. pneumoniae was recalcitrant to gentamicin due to lack of antibiotic penetration. On the other hand, gentamicin could not act on disrupted biofilm cells due to their presence in clusters. However, when depolymerase (20 units/ml) was used in combination with gentamicin (10 [mu]g/ml), dispersal of CPS matrix by enzyme facilitated gentamicin penetration across biofilm. This resulted in significant reduction (p Conclusion Overall the study indicated that, the A. punctata derived depolymerase possesses antibiofilm potential and improves gentamicin efficacy against K. pneumoniae. Moreover, it can serve as a potential substitute to phage borne depolymerases for treating biofilms formed by K. pneumoniae. Keywords: Anti-biofilm enzyme, Bacterial depolymerase, Phage depolymerase, Enzyme kinetics, Gentamicin

Source Citation

Source Citation   

Gale Document Number: GALE|A541372163