High-resolution accurate-mass mass spectrometry enabling in-depth characterization of in vivo biotransformations for intact antibody-drug conjugates

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Date: May 16, 2017
From: Analytical Chemistry(Vol. 89, Issue 10)
Publisher: American Chemical Society
Document Type: Report
Length: 80 words

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Abstract :

The article investigates in vivo biotransformations of multiple site-specific THIOMAB antibody-drug conjugates (TDCs), in the intact form, using a high-resolution, accurate-mass (HR/AM) MS approach. Compared with conventional Q-TOF MS, HR/AM Orbitrap MS enabled more comprehensive identification of antibody-drug conjugates (ADCs) biotransformations. It is particularly beneficial for characterizing ADC modifications with small mass changes such as partial drug loss and hydrolysis. This strategy has significantly enhance the capability to elucidate ADC biotransformations and help understand ADC efficacy and safety in vivo.

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Gale Document Number: GALE|A499579547