miR-153-3p inhibited osteogenic differentiation of human DPSCs through CBF[beta] signaling.

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Date: Apr. 2022
Publisher: Springer
Document Type: Report; Brief article
Length: 325 words

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Keywords: Dental pulp stem cells (DPSCs); Osteogenic differentiation; miR-153-3p; CBF[beta] Abstract Dental pulp stem cells (DPSCs) have multilineage differentiation potential and especially show a great foreground in bone regeneration engineering. The mechanism of osteogenic differentiation of DPSCs needs to be explored exactly. As a kind of endogenous and non-coding small RNAs, microRNAs (miRNAs) play an important role in many biological processes including osteogenic differentiation. However, the mechanism of miR-153-3p in osteogenic differentiation of DPSCs is still unknown. Core-binding factors-beta (CBF[beta]) is a non-DNA-binding factor that combines with the runt-related transcription factor family transcription factors to mediate their DNA-binding affinities, and plays a critical role in regulating osteogenic differentiation. In this study, we explored the mechanisms of miR-153-3p and CBF[beta] in DPSC osteogenesis. The expression of miR-153-3p and CBF[beta] was tested under the osteogenic condition, and the influence led by changing the expression of miR-153-3p or CBF[beta] had also been detected. A luciferase reporter assay confirmed that miR-153-3p directly targeted to CBF[beta]. The osteogenic markers, alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and bone morphogenetic protein 2 (BMP2), were tested in protein level or mRNA level. ALP and Alizarin red staining were used to detect the osteoblast activity and mineral deposition. In osteogenic condition, the expressions of CBF[beta] and osteogenic markers were upregulated, whereas that of miR-153-3p was downregulated. miR-153-3p negatively regulated the osteogenic differentiation, and overexpression of CBF[beta] could offset the negative effect of miR-153-3p. Our findings provided a novel strategy for DPSC application in treatment of bone deficiencies and facilitated bone regeneration. Author Affiliation: (1) The Affiliated Stomatological Hospital of Soochow University, Suzhou Stomatological Hospital, 215000, Suzhou, China (2) Department of Stomatology, Wuxi No 2 People's Hospital, 214000, Wuxi, China (3) Department of Stomatology, Haimen People's Hospital Affiliated To Nantong University, 226199, Nantong, China (4) Department of Stomatology, Affiliated Hospital of Nantong University, 226001, Nantong, China (e) xiao355144377@163.com Article History: Registration Date: 03/16/2022 Received Date: 08/16/2021 Accepted Date: 03/11/2022 Online Date: 04/14/2022 Byline:

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Gale Document Number: GALE|A702795692