The type of anticoagulant used for plasma collection affects in vitro Rhodococcus equi assays.

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From: BMC Research Notes(Vol. 15, Issue 1)
Publisher: BioMed Central Ltd.
Document Type: Report
Length: 3,104 words
Lexile Measure: 1360L

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Abstract :

Objective The efficacy of Rhodococcus equi-specific hyperimmune plasma (HIP) is usually evaluated in vitro. Anticoagulants (AC) used for plasma collection can negatively impact bacterial replication but their effect on R. equi growth has not been evaluated. The aim of this study was to establish the effect that AC routinely used in veterinary medicine (ACD, K.sub.2EDTA, Li Heparin, and Na Citrate) have on in vitro R. equi growth. To assess this, in vitro assays commonly used to test HIP efficacy (direct effect on microorganism and macrophage infection), were performed using each AC and non-treated bacteria. Results There was no direct effect of ACD, Li Heparin or Na Citrate on R. equi growth. These AC significantly (p Keywords: Rhodococcus equi, Prescotella equi, Anticoagulants, Plasma, EDTA, Citrate, Macrophages

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Gale Document Number: GALE|A699458158