How formaldehyde inhibits hydrogen evolution by [FeFe]-hydrogenases: determination by 13C ENDOR of direct Fe-C coordination and order of electron and proton transfers

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From: Journal of the American Chemical Society(Vol. 137, Issue 16)
Publisher: American Chemical Society
Document Type: Report
Length: 86 words

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Abstract :

The article describes the use of formaldehyde (HCHO), a strong electrophile and a rapid and reversible inhibitor of hydrogen production by [FeFe]-hydrogenases, to identify the point in the catalytic cycle at which a highly reactive metal-hydrido species is formed. Study of the reaction of Chlamydomonas reinhardtii [FeFe]-hydrogenase with formaldehyde using pulsed-EPR techniques including electronunuclear double resonance spectroscopy confirmed that formaldehyde binds close to the active site. Moreover, an inhibited super-reduced state having a short Feu13C bond in the 2Fe subsite was supported by density functional calculations.

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Gale Document Number: GALE|A419240637