Occurrence and characterisation of CTX-M enzymes in Enterobacteriaceae isolated from intensive care units of a Turkish University hospital

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Date: October-December 2013
From: Indian Journal of Medical Microbiology(Vol. 31, Issue 4)
Publisher: Elsevier B.V.
Document Type: Article
Length: 579 words
Lexile Measure: 1650L

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Byline: O. Eser, A. Ergin, G. Hascelik

Dear Editor,

We have performed the molecular analysis of extended spectrum beta-lactamases (ESBL) in particular CTX-M genes and antibiotic resistance integrons among Klebsiella pneumoniae , Escherichia coli and Enterobacter spp. strains isolated from patients in intensive care units (ICUs). Randomly selected nonduplicate 117 isolates of E. coli , Klebsiella spp. and Enterobacter spp. were collected from patients admitted to the ICUs of Hacettepe University Hospital through 7 years.

Antimicrobial susceptibility testing was performed by agar dilution method in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. [sup][1] ESBL production was detected by combined disk diffusion. Polymerase chain reaction (PCR) method was used to detect bla [sub]TEM , bla [sub]SHV , bla [sub]CTX-M genes. [sup][2],[3] Sequencing of all positive bla [sub]CTX-M isolates were determined. PCR amplification was performed for the detection of class 1 integron cassettes. [sup][4]...

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Gale Document Number: GALE|A345108450