Byline: O. Eser, A. Ergin, G. Hascelik
We have performed the molecular analysis of extended spectrum beta-lactamases (ESBL) in particular CTX-M genes and antibiotic resistance integrons among Klebsiella pneumoniae , Escherichia coli and Enterobacter spp. strains isolated from patients in intensive care units (ICUs). Randomly selected nonduplicate 117 isolates of E. coli , Klebsiella spp. and Enterobacter spp. were collected from patients admitted to the ICUs of Hacettepe University Hospital through 7 years.
Antimicrobial susceptibility testing was performed by agar dilution method in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines. [sup] ESBL production was detected by combined disk diffusion. Polymerase chain reaction (PCR) method was used to detect bla [sub]TEM , bla [sub]SHV , bla [sub]CTX-M genes. [sup], Sequencing of all positive bla [sub]CTX-M isolates were determined. PCR amplification was performed for the detection of class 1 integron cassettes. [sup]...